Table 1.
Real-time polymerase chain reaction profile of genes involved in nitric oxide signaling and oxidative stress following early ibuprofen or indomethacin treatment in room air or 50% oxygen
| Gene | Sal-O2-E | Ibu-RA-E | Ibu-O2-E | Indo-RA-E | Indo-O2-E |
|---|---|---|---|---|---|
| Genes involved in Regulation of NO signaling pathway | |||||
| CALM1 | -1.2 | -1.1 | -1.2 | -1.6 | 1.0 |
| CAV-1 | -2.0 | -1.9 | -2.9 | 1.1 | -1.3 |
| eNOS | -2.0 | -2.2 | 1.0 | -1.4 | -1.5 |
| iNOS | -1.6 | 1.0 | -1.2 | -1.2 | 1.1 |
| nNOS | 1.1 | 1.5 | 3.9 | 2.7 | -1.2 |
| Genes involved in Regulation of Oxidative Stress | |||||
| CAT | 1.3 | 1.1 | 1.3 | -2.5 | 1.1 |
| GPX1 | -1.5 | -1.4 | -2.8 | 1.3 | -1.9 |
| GPX2 | -1.1 | 1.0 | -1.2 | 1.1 | 1.2 |
| GPX3 | 1.2 | 1.1 | -2.3 | 2.2 | -1.3 |
| GPX4 | 1.1 | 1.1 | 1.1 | 3.7 | -1.1 |
| GPX5 | -2.8 | 1.5 | 3.9 | 2.7 | -1.9 |
| GPX6 | -2.8 | 1.5 | 3.9 | 2.7 | -1.9 |
| GPX7 | -1.6 | -1.3 | -1.7 | 1.4 | -1.5 |
| SOD1 | 1.1 | 1.2 | 1.5 | 1.6 | 1.4 |
| SOD2 | -1.1 | -1.3 | -1.3 | 1.1 | -1.2 |
The data are presented as fold change from Sal-RA-E. All data were corrected using 5 different housekeeping genes. Genes are selected from a profile of 84 genes. In alphabetical order the genes of interest are: CALM1, calmodulin 1; CAT, catalase; CAV-1, caveolin- 1; eNOS, endothelial nitric oxide synthase; GPX1-7, glutathione peroxidase 1-7; iNOS, inducible nitric oxide synthase; nNOS, neuronal nitric oxide synthase; SOD1,2, superoxide dismutase 1,2.