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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nanomedicine (Lond). 2010 Apr;5(3):469–484. doi: 10.2217/nnm.10.12

Figure 2. Fabrication of a 3D cell-laden microscale hydrogels using micromolding.

Figure 2

(A) Liquid prepolymer is deposited onto a hydrophilic poly(dimethyl siloxane) (PDMS) pattern. (B) A hydrophobic PDMS cover slip is placed on top of the prepolymer, forming a reversible seal. (C) Polymer liquid is crosslinked using UV light or heat. (D) The PDMS cover slip is removed. (E) Hydrogels are washed from pattern. (F) Hydrogels are free of the pattern. (G) Flourescent image of microscale tissue constructs comprised of cell-laden hydrogels containing hepatocytes and fibroblasts. Scale bar: 400 μm.

Reproduced with permission from [131].