Table 2.
ICEclc core gene transcript abundance in P. knackmussii B13 cultures grown with 3-chlorobenzoate as a function of growth phase as quantified by macroblot hybridization.
| expo | e-stat | 12 h | 24 h | 36 h | 48 h | 72 h | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Probes | Probe number | mRNAa | Std Devb | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev |
| (%) | (%) | (%) | (%) | (%) | (%) | (%) | |||||||||
| intB13 | 1 | 4.5 | 11.2 | 4.3 | 12.9 | 4.6 | 15.6 | 5.1 | 28.5 | 3.2 | 5.3 | 3.4 | 0.9 | 3.5 | 14.6 |
| ORF52710 | 2 | 21.3 | 46.5 | 29.6 | 8.7 | 17.8 | 3.4 | 9.3 | 39.9 | 7.8 | 53.8 | 12.6 | 18.6 | 6.4 | 41.8 |
| ORF53587 | 3 | 4.2 | 30.2 | 2.9 | 25.9 | 2.6 | 27.1 | 1.7 | 37.3 | 3.4 | 11.9 | 3.1 | 20.4 | 1.4 | 12.2 |
| ORF59888 | 4 | 18.6 | 33 | 20 | 7.5 | 14.7 | 18.9 | 8.4 | 32.3 | 16.8 | 23.9 | 22.4 | 9.3 | 14.6 | 43.4 |
| ORF65513 | 5 | 17.3 | 19.4 | 17.1 | 0.8 | 16.7 | 10.3 | 13.4 | 9.9 | 11.8 | 9.5 | 13.5 | 2.4 | 12.4 | 10.7 |
| ORF67800 | 6 | 16.6 | 2.7 | 12.4 | 26.1 | 10.1 | 11.6 | 8 | 12.9 | 14.6 | 4.3 | 12.6 | 10.7 | 8.5 | 16.6 |
| ORF68987c | 7 | 2.1 | 4.3 | 1.7 | 8.2 | 1.2 | 30.1 | 0.8 | 12.9 | 1.7 | 6.5 | 1.5 | 4.3 | 1 | 22.3 |
| ORF73029 | 8 | 2.5 | 20.8 | 1.4 | 15 | 2.1 | 18.5 | 2.6 | 15 | 2 | 14.6 | 2.2 | 2.3 | 1.5 | 10.4 |
| ORF75419 | 9 | 7.5 | 18.1 | 4.5 | 7.6 | 8.7 | 0.4 | 11.1 | 32 | 14 | 27.1 | 20.5 | 9.4 | 28 | 31.6 |
| ORF81655 | 10 | 10.2 | 30.1 | 6.4 | 35.8 | 104 | 4.8 | 168 | 24.5 | 113 | 24.3 | 191 | 14.5 | 177 | 10.9 |
| ORF83350 | 11 | 3.3 | 18.9 | 1.7 | 7 | 0.9 | 17.8 | 0.9 | 26.1 | 0.9 | 3.5 | 0.9 | 5 | 0.9 | 5.6 |
| ORF84835 | 12 | 0.4 | 14.4 | 0.3 | 38.3 | 7.4 | 53.2 | 9.5 | 7.7 | 20.6 | 28.9 | 28.1 | 29.5 | 29.8 | 22.8 |
| ORF87986 | 13 | 5 | 1 | 4.6 | 39.3 | 41.1 | 16.1 | 64.5 | 7.2 | 41.3 | 12.9 | 54.9 | 22.1 | 100 | 11.5 |
| ORF89746 | 14 | 12.9 | 34.1 | 12.9 | 10.4 | 4.9 | 12 | 2.2 | 41.2 | 1.3 | 44.9 | 3.4 | 28.9 | 3.2 | 19.8 |
| ORF91884 | 15 | 3.3 | 11.7 | 2.1 | 25.6 | 2.4 | 14.7 | 3 | 32.4 | 3.1 | 14 | 3.2 | 11.3 | 4.1 | 5.2 |
| inrR | 16 | 8.3 | 11.9 | 6.4 | 10.5 | 7.4 | 13.1 | 4.5 | 11.6 | 5.1 | 18.8 | 7.2 | 0.6 | 5.9 | 12 |
| ORF96323 | 17 | 3 | 13 | 1.7 | 13.3 | 1.1 | 5.5 | 1 | 2.8 | 1.3 | 12.8 | 1.1 | 14.4 | 1.2 | 13.2 |
| ORF98147 | 18 | 1.1 | 10.7 | 0.5 | 14.2 | 0.4 | 4.6 | 0.5 | 5.9 | 0.5 | 8.3 | 0.4 | 2.4 | 0.4 | 0.9 |
| ORF100033 | 19 | 30.6 | 4 | 23 | 4.3 | 26 | 8.7 | 12.3 | 16.7 | 19.6 | 14.3 | 20.4 | 22.4 | 21.5 | 16.3 |
| ORF100952 | 20 | 1.4 | 13.2 | 3.7 | 31.2 | 2.8 | 4.9 | 1.8 | 3.1 | 1.7 | 58.5 | 3.5 | 30.8 | 6.7 | 13.4 |
| ORF101284 | 21 | 3.7 | 18.9 | 4.5 | 10.1 | 2.1 | 5.8 | 1 | 23.1 | 1.9 | 9.7 | 1.5 | 11 | 1.3 | 11.5 |
a) mRNA is the average calculated amount of mRNA copies × 108 per μg of total RNA from triplicate determinations. This value corresponds approximately to the calculated number of mRNA of this transcript per cell. Expo, exponential phase; e-stat, early stationary phase (6 h).
b) standard deviation from the average mRNA, expressed in percentage.
c) ORFs surrounded by a common border are on the same transcript.