Table 3.
Quantification of ICEclc core gene expression by dot-blot hybridization in strain B13 grown on different carbon substrates.
| Exponential phase | After 24 h at stationary phase | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3-chlorobenzoate | succinate | 3-chlorobenzoate | succinate | fructose | glucose | |||||||
| Probe number and probe | mRNAa | Std Devb | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev | mRNA | Std Dev |
| (%) | (%) | (%) | (%) | (%) | (%) | |||||||
| 1) intB13 | 4.5 | 11.2 | 7.3 | 13.1 | 5.1 | 28.5 | 4.1 | 11.2 | 4.4 | 51.7 | 3.2 | 8.1 |
| 2) ORF52710 | 21.3 | 46.5 | 19.7 | 16.9 | 9.3 | 39.9 | 9.6 | 8 | 5.1 | 42.7 | 9.4 | 30.6 |
| 3) ORF53587 | 4.2 | 30.2 | 3.6 | 0.1 | 1.7 | 37.3 | 1.7 | 21.1 | 2 | 0.4 | 1.9 | 2.6 |
| 4) ORF59888 | 18.6 | 33 | 16.9 | 2.3 | 8.4 | 32.3 | 12.9 | 18.6 | 16.8 | 7.3 | 23.8 | 15.9 |
| 5) ORF65513 | 17.3 | 19.4 | 19.5 | 2.8 | 13.4 | 9.9 | 12.7‡ | 5.3 | 13.8 | 7.6 | 13.8 | 11 |
| 6) ORF67800 | 16.6 | 2.7 | 16.6 | 5.5 | 8 | 12.9 | 12.7 | 18.3 | 11.6 | 33.7 | 17.9 | 38.6 |
| 7) ORF68987 | 2.1 | 4.3 | 2.1 | 11 | 0.8 | 12.9 | 0.8 | 0.2 | 1.3 | 13.8 | 1.1 | 11.7 |
| 8) ORF73029 | 2.5 | 20.8 | 2.9 | 12.6 | 2.6* | 15 | 0.9‡ | 18.2 | 1.4 | 6.7 | 1.1 | 4.2 |
| 9) ORF75419 | 7.5 | 18.1 | 7.3 | 6.8 | 11.1 | 32 | 3‡ | 3.9 | 3.9 | 3.3 | 2.8 | 5.4 |
| 10) ORF81655 | 10.2 | 30.1 | 18.7 | 36.6 | 168* | 24.5 | 6.3 | 2.7 | 45.7* | 3.6 | 9.2 | 27 |
| 11) ORF83350 | 3.3 | 18.9 | 2.8 | 16.5 | 0.9 | 26.1 | 0.5 | 37.3 | 0.5 | 14.5 | 0.4 | 10.2 |
| 12) ORF84835 | 0.4 | 14.4 | 0.3 | 16.3 | 9.5* | 7.7 | 0.3 | 25.8 | 1.7 | 16.1 | 0.3 | 1.5 |
| 13) ORF87986 | 5 | 1 | 5.2 | 0.1 | 64.5* | 7.2 | 5.5‡ | 0.4 | 14.2 | 26.9 | 5.5 | 2 |
| 14) ORF89746 | 12.9 | 34.1 | 24.4 | 19.8 | 2.2 | 41.2 | 2.1 | 17.3 | 2.1 | 36.7 | 0.5 | 15.4 |
| 15) ORF91884 | 3.3 | 11.7 | 4.5 | 3 | 3 | 32.4 | 1.6‡ | 3.2 | 2.3 | 33.1 | 1.1 | 5.9 |
| 16) inrR | 8.3 | 11.9 | 8.2 | 21.3 | 4.5 | 11.6 | 4 | 7.5 | 6.4 | 8.1 | 4.9 | 39.7 |
| 17) ORF96323 | 3 | 13 | 5.3 | 27.1 | 1 | 2.8 | 1.8 | 35.6 | 0.9 | 53.2 | 1.1 | 31.8 |
| 18) ORF98147 | 1.1 | 10.7 | 1.5 | 5.1 | 0.5 | 5.9 | 0.4‡ | 7 | 0.4 | 3.7 | 0.4 | 1.7 |
| 19) ORF100033 | 30.6 | 4 | 40.4 | 20.2 | 12.3 | 16.7 | 17.6 | 18 | 22.9 | 6.4 | 22.2 | 30.2 |
| 20) ORF100952 | 1.4 | 13.2 | 2.2 | 22.2 | 1.8§ | 3.1 | 0.9 | 1.7 | 1.9 | 7.9 | 0.9 | 29.4 |
| 21) ORF101284 | 3.7 | 18.9 | 3.2 | 6.9 | 1 | 23.1 | 1‡ | 7.9 | 1.1 | 1.9 | 1.2 | 14.5 |
a) mRNA is the average calculated amount of mRNA copies × 108 per μg of total RNA from triplicate determinations. This value corresponds approximately to the calculated number of mRNA of this transcript per cell.
b) standard deviation from the average mRNA, expressed in percentage.
c) *, values statistically significantly different to the cells cultivated with other carbon sources. §, values statistically significantly different to the cells cultivated with succinate or glucose. ‡, values statistically significantly different between exponential phase and stationary phase. P ≤ 0.05 in pairwise Student's T test.