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. 2010 Jun 25;5(6):e11322. doi: 10.1371/journal.pone.0011322

Figure 7. Inhibition of transcellular ion pathways modulates TER in sham not 2CLP monolayers.

Figure 7

Following initial TER measurements, the apical fluid was replaced with 1mM amiloride (N = 9) or the basal fluid replaced with 1mM ouabain (N = 10) to block Na+K+-ATPase and ENaC activity. DMEM was used as a control media (N = 8). Ouabain, not amiloride, treatment of sham wells resulted in significant increases above DMEM controls (-). Neither treatment had a significant affect on TER in 2CLP wells. Significance defined as p<0.05.