Figure 8. Expression levels of the TJ proteins claudin 3, 4, 5, 7, 8, 18, ZO-1, Occludin, and JAM-A were determined via western blot in sham (S) and 2CLP (C) monolayers.

Significant reductions in claudin 4, 18, and occludin were observed (p<0.05, N≥3). Representative Western blots of all proteins analyzed are show along with their respective actin bands to which they were normalized. (mean ± SE).