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. 2010 Jun 25;5(6):e11327. doi: 10.1371/journal.pone.0011327

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Cell-free ROka-LacZ was pre-incubated with rIDE or BSA at 25°C for the indicated time before infecting MeWo cells. The ratio of the number of blue foci in cells infected with virus treated with rIDE or BSA(control) was determined. The experiment was preformed 11 times and a representative experiment is shown. Vertical lines indicate standard deviations. (B) Pre-treatment of VZV with rIDE at 4°C enhances viral infectivity. Cell-free ROka-GFP virus was incubated with medium, BSA, rIDE or denatured rIDE (rIDE treated with acid followed by base to a final neutral pH) for 60 min at 4°C before infecting cells. (P = 0.001 for rIDE vs. denatured rIDE, Student t test). Vertical lines indicate standard deviations. The experiment was performed 3 times, and a representative result is shown.