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kdrl:GFP embryos were injected at the one-cell stage with control morpholino oligonucleotide (MO), pdgfrβ2 MO, or pdgfrβ2 MO plus pdgfrβ2 mRNA, and analyzed for angiogenic sprouting at 24 hpf in the presence of DMSO control or 100 nM of the PI3 kinase inhibitor Wortmannin. *** indicates p<0.001. All data represent the mean +/− standard error.
