Skip to main content
. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Circ Res. 2010 Apr 22;106(12):1818–1828. doi: 10.1161/CIRCRESAHA.110.217950

Fig 3.

Fig 3

Expansion of tbx18 expression in the cardiac region by overexpression of bmp2b or tcf21. (A–C) Embryos from a hemizygous Tg(hsp70:bmp2b)fr13 outcross were heat-shocked at 36 hpf, and harvested and analyzed at 57 hpf. The expression of tbx18 in the cardiac region was expanded (19 expanded/25 scored, arrow in C points to ectopic tbx18 expression) in the heat shocked transgenic embryos (B, C) compared to their heat shocked non-transgenic siblings (A). (The same embryo is shown in (B) and (C), but at different focal planes.) (E–G) 20 pg of tcf21 mRNA was injected into one-cell stage wildtype (E, F) or acvr1l mutant (G) embryos, which were harvested at 57 hpf and processed for whole mount in situ hybridization for tbx18. Overexpression of tcf21 mRNA significantly expanded tbx18 expression (arrows) in the cardiac region of wildtype embryos (16 increased/25 scored) (E, F) but was not able to rescue the loss of tbx18 expression in acvr1l mutant embryos (G) compared to wildtype (D).