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. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Circ Res. 2010 Apr 22;106(12):1818–1828. doi: 10.1161/CIRCRESAHA.110.217950

Fig 5.

Fig 5

Bmp4 is required for proepicardial cell specification. (A–B) whole mount in situ hybridization of 48 hpf embryos for bmp4 (A) and bmp2b (B). bmp4 is expressed in the cardiac outflow tract, atrioventricular myocardium (between arrowheads) and inflow tract (arrow) regions (A), whereas bmp2b (B) does not appear to be expressed in the cardiac region around 48 hpf. (C–F) bmp4 mutant embryos and their wildtype siblings were harvested at 57 hpf and processed for in situ hybridization for tbx18 (C, D) or tcf21(E, F). tbx18 (D) and tcf21 (F) expression in the cardiac region is severely reduced in bmp4 mutants (19 reduced/21 scored for tbx18, and 16 reduced/17 scored for tcf21) compared to their wildtype siblings (C, E).