Fig. 1.

QC expression in mouse Edinger–Westphal nucleus. Top row QC immunoreactivity is absent from midbrain sections of QC knock-out mice as shown at increasing magnifications from left to right. In contrast, there is a specific and robust labelling of a neuronal population in the EWN of wild type mice (middle row). Since the EWN is known to contain both, Ucn-1 and ChAT neurons, double labellings were performed. QC (green fluorescence) was found to be co-localised with Ucn-1 (red fluorescence; bottom row), but not with ChAT (not shown)