Fig. 5.

DLS titrations of β-lac (a) and BSA (b) with the following polymers: open circle P2MVPI₄₃, inverted closed triangle P2MVPI₂₂₈, open triangle P2MVPI₄₁-b-PEO₂₀₄, closed circle PAA₂₁-b-PAPEO₁₄. The initial concentration of protein and the initial concentration of the polymer titrant were 100 mg/l. Titrations were carried out in 10 mM NaCl, pH 7 ± 0.1. For P2MVPI₄₃, P2MVPI₂₂₈, and P2MVPI₄₁-b-PEO₂₀₄, f- on the abscissa is defined as [protein-]/([protein-] + [P2MVPI+]), and for PAA₂₁-b-PAPEO₁₄ f- is defined as [protein-]/([protein-] + [PAA-]). Ordinate: intensity I [kilohertz, au] divided by the total protein concentration c [grams per liter]. For calculations, we assumed that under experimental conditions, BSA has 13 negative charges per molecule [35] and β-lac has 13 negative charges per dimer [36]