. Author manuscript; available in PMC: 2010 Jun 27.

Published in final edited form as: Proteomics. 2009 Oct;9(20):4632–4641. doi: 10.1002/pmic.200900398

Table 2.

Strategies for enriching PTM peptides

Method	Case studies	Advantages	Disadvantages
Antibody	^AcR or ^MeK, pTyr,	High speed and specificity	Require good antibody
Metabolic tagging	O-GlcNAc, farnesylation		Artificial system, limited to bulk PTMs
In vitro derivatization	pSer/Thr, O-GlcNAc, glycoproteins	Reagent accessible	Multiple reactions, reduced sensitivity, side reactions
Chemoenzymatic	O-GlcNAc	Reagent accessible	Multiple steps
Ionic interaction based	pSer/Thr/Tyr	Established method
Other affinity reagents	Glycoproteins/lectin	Reagents accessible	Low-binding affinity

^AcR, ^MeR, pSer, pThr, pTyr represent acetylated arginine, methylated arginine, phosphorylated serine, threonine, and tyrosine residues, respectively.