Table 2.
Percentage of sequence identity between pairs of nucleotide (Nucl), or deduced amino acid (AA) sequences), or between substrate recognition site (SRS) regions, as determined after optimal pair-wise alignment with ClustalW in BioEdit (Hall, 1999). Comparisons were made between the cloned rainbow trout CYP1B1 CYP1C1 CYP1C2, and CYP1C3 (coding sequence), and between the cloned sequences, and orthologous sequences in zebrafish and human. Accession numbers are available in the supplemental information. Bold underlined numbers indicate the highest identities observed.
| Rainbow trout | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CYP1B1 | CYP1C1 | CYP1C2 | CYP1C3 | ||||||||||
| Nucl | AA | SRS | Nucl | AA | SRS | Nucl | AA | SRS | Nucl | AA | SRS | ||
| Rainbow trout | CYP1C1 | 56 | 47 | 56 | |||||||||
| CYP1C2 | 55 | 48 | 57 | 85 | 84 | 82 | |||||||
| CYP1C3 | 56 | 48 | 57 | 94 | 93 | 89 | 83 | 82 | 82 | ||||
| Zebrafish | CYP1B1 | 66 | 64 | 79 | 54 | 48 | 58 | 56 | 50 | 58 | 55 | 49 | 56 |
| CYP1C1 | 55 | 48 | 58 | 75 | 82 | 85 | 73 | 79 | 81 | 75 | 82 | 86 | |
| CYP1C2 | 55 | 47 | 56 | 70 | 73 | 74 | 72 | 79 | 86 | 70 | 72 | 73 | |
| Human | CYP1B1 | 57 | 50 | 58 | 55 | 48 | 57 | 59 | 47 | 55 | 55 | 47 | 55 |