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In situ hybridization of chicken embryos with spalt4 RNA probe. A. Stage 10 embryo showing signal in the otic placode (arrowhead) and presomitic mesodem (arrow). B. Stage 14 embryo showing signal in the otic pits (black arrowhead) and midbrain (arrow). The region that will give rise to the epiphysis also expresses spalt4 (white arrowhead). Other forebrain staining is background.
