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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Dev Biol. 2009 Nov 10;340(2):222–231. doi: 10.1016/j.ydbio.2009.11.004

Figure 8.

Figure 8

Pea3 and Pax2 can induce CR-F activity in the extraembryonic ectoderm and together can induce CR-F activity throughout the embryo. Embryos co-electroporated with Pea3pCIG and CR-F12 cherry (A,B), Pax2pCIG and CR-F12 Cherry (C,D) and Pea3pCIG, Pax2pCIG and CR-F12 cherry (E–H). A. Pea3 expressed throughout the embryo. B. Cherry driven by CR-F was expressed in the otic placode and in the extraembryonic ectoderm (arrohead). C. Pax2 was expressed throughout the embryo. D. Cherry driven by CR-F was expressed in the otic placode and the extraembryonic ectoderm (arrowhead). E. G. combined expression of Pea3 and Pax2 throughout the ectoderm. F. H. Cherry driven by CR-F was detected in the trunk ectoderm (arrow) as well as the extraembryonic ectoderm (arrowhead). (E) and (F) are lower magnification pictures of the embryo in (G) and (H). The otic placode is outlined in (B), (D), (F), and (H).