Fig. 9.

Effect of perlecan modification by peroxynitrite on Baf-32 cell proliferation and binding of FGF-2. (A) BaF3 cells (expressing FGF receptor 1c) were incubated with perlecan and FGF2 and the relative amount of growth was measured as described in the methods section. (B) Surface absorbed perlecan (32 nM, black bars) was exposed to peroxynitrite (ONOO, 32 μM, white bars) or dONOO (32 μM, light grey bars) in 0.1 M phosphate buffer, pH 6.5 or pH 7.5 for 20 min at 22 °C. The extent of [¹²⁵I]-labeled FGF-2 binding after 2 h incubation at 22 °C was quantified by gamma counting. a significantly different to untreated perlecan control.