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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Haematologica. 2007 Jul 20;92(8):1013–1020. doi: 10.3324/haematol.11407

Table 1.

Primers used during TERC plasmid mutagenesis

TERC mutation 1 Sequence 2
c.180C→T (C180T) caaacaaaaaatgtTagctgctggcccgt
acgggccagcagctAacattttttgtttg
c.112C→T (C112T)
complement to c.178G?A		 gtttttctcgctgaTtttcagcgggc
gcccgctgaaaAtcagcgagaaaaac
c.110G→A (G110A)
complement to c.180C?T		 gtttttctcgctAactttcagcgggc
gcccgctgaaagtTagcgagaaaaac
c.2G→C (G2C) gcagcgcaccgCgttgcggagggt
accctccgcaacGcggtgcgctgc
1

Nucleotide number in TERC gene where G of the initial RNA sequence GGG is depicted as number 1

2

Primer sequence 5′→3′ with the forward primer above the reverse primer. The mutated base in each primer is highlighted in bold.