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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Cell. 2009 May 15;137(4):697–707. doi: 10.1016/j.cell.2009.04.044

Figure 6.

Figure 6

Recruitment of SMC to an ectopic site impairs chromosome organization and DNA segregation. DNA was visualized by DAPI (false-colored green) and membranes were visualized with FM4-64 (red). Wild-type cells have compact nucleoids that form bi-lobed structures during DNA replication and often segregate prior to cell division. In the absence of the eight parS sites (ΔparS), the nucleoids are less compact. Insertion of an array of 16 parS sites in a strain lacking the origin-proximal parS sites causes an increase in production of anucleate cells (yellow carets), cell divisions on top of the DNA (white caret) and aberrant nucleoid morphology.