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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Traffic. 2008 Jul 9;9(9):1551–1562. doi: 10.1111/j.1600-0854.2008.00786.x

Figure 1. The cytosolic tails of LRP9 and LRP12 contain 2 AC-LL signals.

Figure 1

Figure 1

(A) The partial amino acid sequences of the cytosolic tails of LR3, LRP9 and LRP12 are shown from the end of the transmembrane (TM) segments to the C-termini of the proteins. (B) Sequences of the various AC-LL signals used in this study. Proteins were expressed as GST-fusions and purified from E. coli BL21 cells. (C) Schematic representation of wild-type and mutant LRP9 and LRP12 constructs used in the immunofluorescence and cell-surface biotinylation studies. For endocytosis assays, mLRP4-LRP9 chimeric constructs were made by fusing the fourth ligand binding and transmembrane domains of LRP1 with the wild type or mutant cytoslic tails of LRP9.