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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Traffic. 2008 Jul 9;9(9):1551–1562. doi: 10.1111/j.1600-0854.2008.00786.x

Figure 2. LRP9 and LRP12 contain an internal GGA binding sequence in addition to a carboxy-terminal DXXLL motif.

Figure 2

Figure 2

(A-F) Pull down assays were performed using GST fusions encoding the indicated AC-LL signals of the different LRPs with Sf9 cell-expressed GGAs (A, C, D, and E-left panel), or GST-GGA2 VHS with HEK 293 cell-expressed wild-type and mutant LRPs and LERP (B, E-right panel, and F). Ponceau staining of the membrane (A, D, and E-left panel) indicates that similar amounts of the various GST-fusions were used.