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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Methods Mol Biol. 2009;490:165–178. doi: 10.1007/978-1-59745-367-7_7

Fig. 7.4.

Fig. 7.4

Urea titrations of FlAsH-labeled protein in vitro as a function of the incubation time (monitored by Trp fluorescence): (A) tetra-Cys CRABP and (B) P39A tetra-Cys CRABP. The actual urea concentration was determined by measuring the refractive index, and data are curve-fit to a two-state model. (Reproduced from Ref. (12) with permission from Willey InterScience.)