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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Methods Mol Biol. 2009;490:165–178. doi: 10.1007/978-1-59745-367-7_7

Fig. 7.5.

Fig. 7.5

Reversibility of the in vivo urea titrations. FlAsH-labeled tetra-Cys CRABP-expressing cells were treated with 3 M urea for 75 min, and then refolding was initiated by dilution of aliquots into fresh LB medium containing 100 μg/mL ampicillin. After incubation of the cells for 60 min, the extent of return of the FlAsH signal, as a measure of refolding, was monitored by FlAsH fluorescence (open symbols). A urea melt of FlAsH-labeled tetra-Cys CRABP incubated for 75 min is given for comparison (closed symbols).