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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Pharmacol Biochem Behav. 2010 Mar 15;95(4):410–421. doi: 10.1016/j.pbb.2010.02.019

Figure 4.

Figure 4

Effects of CTAP pretreatment on METH-induced zif/268 mRNA expression in the rostral CPu. In situ hybridization film autoradiograms showing zif/268 (A) and mu opioid receptor immunohistochemical staining in adjacent sections (B) and quantitative analysis of PD mRNA expression in the patch and matrix compartments of DL (C), DM (D), VM (E) and VL (F) CPu, from rats intrastriatally infused with vehicle or CTAP (10 μg/μl), 15 min prior to METH or saline treatment. Arrows indicate patches of mu opioid receptor labeling and corresponding patches of zif/268 mRNA expression. Note the decrease in zif/2678 expression in METH-treated animals pretreated with CTAP. Quantitative values are average gray values (arbitrary units; ±S.E.M., n=5–8 animals/group) obtained from densitometric analysis of film autoradiograms. *Significantly different from saline-pretreated control group, P<0.05; +Significantly different from respective saline-pretreated group, P<0.05. There was not a significant main effect of METH treatment in the VL CPu.