Skip to main content
. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Dev Biol. 2008 Jun 6;321(2):446–454. doi: 10.1016/j.ydbio.2008.05.551

Fig. 2. Dissection of the UpE and IntE regions reveals WREs that contain functional TCF binding sites.

Fig. 2

(A) When cloned upstream of a hsp70 core promoter/luciferase reporter, UpE activated luciferase expression when co-expressed with Arm*. UpE was divided into three fragments (#1 – 3), none of which were highly responsive to Arm*. However, two overlapping stretches (UpE1 and UpE2) possessed strong WRE activity. Vertical lines in the boxes represent the predicted TCF sites that are conserved in 12 Drosophila species, while the dotted lines denote mutated TCF sites. UpE1 and UpE2 both require a subset of the TCF sites for Arm* responsiveness. (B) The IntE genomic region contains a 255 bp WRE that requires three TCF binding sites for Arm* responsiveness. Fold activation is relative to luciferase expression without Arm* expression for each reporter construct. Each result is the mean of triplicate transfections, with the standard deviation indicated in parenthesis. The data shown are a representative example from more than three separate experiments.