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. 2010 Jun 25;5(6):e11318. doi: 10.1371/journal.pone.0011318

Figure 2. Evidence for Lewis acid complexation and improved borate electrophoresis at pH 6.5.

Figure 2

DNA fragments of 4 kb, 1 kb and 200 bp are marked by arrowheads approximating the leading edges of the bands, and the 12 kb band is marked at the center of the lagging edge. The sizes of all fragments of the ladder were, in bp, 100, 200, 300, 400, 500, 650, 850, 1000, 1650, and 2000 through 12,000 in 1000-bp increments. (a) Electrophoretic separation for anions of differing Lewis acid character. (b) Separation using borates at different pHs, given a constant migration for the 200 bp band. (c) Separation (in 2% agarose) of bands from 100 bp to 1000 bp in 6.5 pH borate.