Table 2.

Sense oligonucleotides used for EMSA, the competition and mutation analysis in EMSA and for the construction of mutant plasmids.

Mutated oligonucleotides (^mut) are listed below wild-type oligonucleotides and mutation sites are highlighted through vertical lines. Mutations were created directly between the nucleotides −93 and −56 at positions −78 and −77 (CP-93/-56CATm) or between the nucleotides −1255 and −1213 at positions −1225 and −1224 (CP-1255/1213CAT5m). The mutation oligonucleotide CP -93/-56 M10-12 was created by replacing the CGGA motif of the wildtype oligonucleotide with a TACA motif in the mutated sequence.

The deletion oligonucleotides (^del) CP -93/-56 D9 and CP -93/-56 D13 were generated by removal of the 3′ region from the wild-type oligonucleotide CP -93/-56 D16. Oligonucleotides cp-1255/-1234 and cp-1233/-1213 were used for competition studies (^comp).

Lower cases indicate mutated nucleotides. The corresponding wild-type sequences are marked in bold characters. These mutations were used for EMSA competition assays and for the generation of mutant plasmids. The CCAAT box was underlined.