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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Differentiation. 2008 Oct 25;77(1):29–37. doi: 10.1016/j.diff.2008.09.005

Figure 3.

Figure 3

Immunoreactivity of HODCs with neural markers. Early passage (passages 2 and 3) HODCs immunoreacted positively with markers of early neural stem cell progenitors that included nestin (A) and Sox-2 (B), and a late marker of neural differentiation, βIII-tubulin. Comparatively, both DPSCs and maxilla BMSCs only immunoreacted with nestin (D and E respectively). Quantitative analysis by real time-PCR showed a significantly much higher nestin expression (P< 0.04) by HODCs than either DPSCs or maxilla BMSCs (F). There was no statistically significant differences between DPSCs and BMSCs. Primary anti-human Sox-2 was labeled with AlexaFluor 488 goat-anti-mouse secondary antibody while primary anti-human nestin and anti-human βIII-tubulin were labeled with AlexaFluor 555 goat anti-mouse secondary antibody. [GAPDH = glyceraldehyde-3-phosphate dehydrogenase].