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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Biochemistry. 2007 Dec 15;47(1):167–182. doi: 10.1021/bi701954p

TABLE 6.

Steady-state kinetic constants for mutant PA4872 catalyzed decarboxylation of oxaloacetate.a

kcat (s−1) Km (μM) kcat/Km (M−1s−1)
WT 7500 ± 200 2200 ± 100 3.4 × 106
Y212F 323 ± 6 87 ± 4 3.7 × 106
H235A 510 ± 7 1020 ± 50 5.0 × 105
H235Q 2580 ± 90 2500 ± 200 1.0 × 106
a

0.25 mL reaction mixture contained 50 mM K+Hepes (pH 7.5, 25 °C), 5 mM MgCl2, 0.5-10 Km of oxaloacetate. Reactions were monitored at 255 nm.