FIGURE 5.
Ablation of striatal NK-1R-expressing interneurons does not prevent METH-induced depletion of TH. The mice (n = 6) received an intrastriatal injection of SSP-SAP into one side and SAP into the contralateral side. Then, 3 weeks later, the mice received a single injection of METH (30 mg/kg, i.p.) and were sacrificed 3 days after METH. Striatal sections were processed for NK-1R and TH immunofluorescence in the same section. Note that SSP-SAP abolishes NK-1R staining without affecting staining for TH (b and d). NK-1Rs were visualized with an antibody conjugated to FITC (a, b, e & f) and TH with Cy3 (c, d, g & h).