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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: J Immunol. 2008 Dec 15;181(12):8258–8266. doi: 10.4049/jimmunol.181.12.8258

Figure 4. Germinal center formation in ICOS-/- mice is largely abrogated in response to either Pn14 or conjugate.

Figure 4

WT (C57BL/6) and ICOS-/- mice (4 mice per group) were immunized i.p. with either Pn14 in saline or PS14-PspA + C-PS-PspA in alum/CpG-ODN. Spleens were removed on day 10 and stained with PNA-Alexa Flour 488 (green), anti-B220-Pacific Blue (blue), and anti-CD4-PE (red). Four sections from each of 4 spleens per group were analyzed for GC number and area for a total spleen area of 12mm2 (see “Results”).