Table 1. Quantitation of splenic and peritoneal B cell subsets in WT, SAP-/-, ICOS-/-, and CD28-/- mice.
Spleen and peritoneal cells from individual mice (WT, SAP-/-, and ICOS-/- mice [three per group]; CD28-/- mice [4 mice]) were analyzed by flow cytometry as indicated in “Materials and Methods”. Data is expressed as arithmetic mean +/- SEM.
| |
Total cells (× 106) |
% (No. ×106) of cells in subset |
||
|---|---|---|---|---|
| FB |
MZB |
B-1 |
||
| Spleen | ||||
| WT | 69.16 ± 1.02 | 27.48 ± 0.03 (19.00 ± 0.02) | 3.78 ± 0.15 (2.61 ± 0.10) | 3.43 ± 0.05 (2.37 ± 0.03) |
| SAP-/- | 65.89 ± 8.90 | 32.55 ± 1.06* (21.45 ± 0.70*) | 3.75 ± 0.04 (2.47 ± 0.02) | 3.45 ± 0.04 (2.27 ± 0.02*) |
| ICOS-/- | 75.04 ± 14.29 | 37.70 ± 0.20* (28.29 ± 0.15*) | 4.16 ± 0.01* (3.12 ± 0.01*) | 3.57 ± 0.05 (2.68 ± 0.04*) |
| CD28-/- | 69.98 ± 1.18 | 26.83 ± 1.15 (18.78 ± 0.80) | 3.72 ± 0.25 (2.59 ± 0.17) | 3.80 ± 0.18 (2.66 ± 0.13) |
| |
% (No. ×106) of cells in subset |
|||
|---|---|---|---|---|
| B-1a |
B-1b |
B-2 |
||
| Peritoneum | ||||
| WT | 5.31 ± 0.41 | 3.19 ± 0.63 (0.17 ± 0.03) | 3.30 ± 0.85 (0.18 ± 0.05) | 3.25 ± 0.75 (0.17 ± 0.04) |
| SAP-/- | 6.26 ± 3.86 | 2.71 ± 1.33 (0.17 ± 0.08) | 6.60 ± 0.66* (0.41 ± 0.04*) | 6.39 ± 1.17* (0.40 ± 0.07*) |
| ICOS-/- | 2.62 ± 0.41 | 3.40 ± 1.17 (0.09 ± 0.03) | 2.75 ± 0.51 (0.07 ± 0.01*) | 9.00 ± 1.17* (0.24 ± 0.03) |
| CD28-/- | 5.44 ± 0.96 | 6.78 ± 0.88* (0.37 ± 0.05*) | 5.22 ± 0.71 (0.28 ± 0.04) | 3.85 ± 0.07 (0.21 ± 0.01) |
*
significance (p<0.05) relative to WT mice, by Student t test.