Table 2. Characteristics of primers, probes, and PCR efficiencies of the housekeeping and target genes used in this study.
| Gene | Oligo | Sequence | Amplicon (bps) | PCR efficiency |
|---|---|---|---|---|
|
GAPDHNM_008084
|
F |
TGTGTCCGTCGTGGATCTGA |
77
|
91.3%
|
| R |
CCTGCTTCACCACCTTCTTGA |
|||
| P |
CCGCCTGGAGAAACCTGCCAAGTATG |
|||
|
LDHANM_010699
|
F |
ATCCCATTTCCACCATGATT |
183
|
86%
|
| R |
ACTGCAGCTCCTTCTGGATT |
|||
| P |
CAGGCGGGCCTCTTCCTCAG |
|||
|
ACTBNM_007393
|
F |
GCAAGCAGGAGTACGATGAG |
148
|
92.7%
|
| R |
CCATGCCAATGTTGTCTCTT |
|||
| P |
TCCATCGTGCACCGCAAGTG |
|||
|
UBCNM_019639
|
F |
ACCAGCAGAGGCTGATCTTT |
110
|
88.7%
|
| R |
ACCTCTGAGGCGAAGGACTA |
|||
| P |
CTGGAAGATGGCCGCACCCT |
|||
|
PPIANM_008907
|
F |
AATGCTGGACCAAACACAAA |
117
|
100.3%
|
| R |
TTCCACAATGTTCATGCCTT |
|||
| P |
TGCTTGCCATCCAGCCATTCA |
|||
|
TBP1NM_013684
|
F |
ATCAACATCTCAGCAACCCA |
187
|
97.1%
|
| R |
TTGAAGCTGCGGTACAATTC |
|||
| P |
ACCACTGCACCGTTGCCAGG |
|||
|
HPRT1NM_013556
|
F |
GGCCAGACTTTGTTGGATTT |
155
|
95.1%
|
| R |
CAGATTCAACTTGCGCTCAT |
|||
| P |
TGACACAAACGTGATTCAAATCCCTG |
|||
|
RPL5NM_016980
|
F |
GGAAGCACATCATGGGTCAGA |
70
|
91.8%
|
| R |
TACGCATCTTCATCTTCCTCCATT |
|||
| P |
TGTGGCAGACTACATGCGCTACC |
|||
| TktNM_009388 | F |
GACAGTGCCCTTCTGCAGTACTT |
65 | 101.1% |
| R |
CCATGCGAATCTGGTCGAA |
|||
| P | CGCGGCCTTCTTCACACGGG |
F, forward primer; R, reverse primer; P, Probe, labeled as 5'-FAM, 3'-TAMRA.