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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Arthritis Rheum. 2005 Mar;52(3):800–809. doi: 10.1002/art.20937

Figure 3.

Figure 3

Electrophoretic mobility shift assay (EMSA) analysis of RAR (A), RXR (B), and Sp-1 (C). EMSA reactions were performed using biotin-labeled probes that were incubated with 25 μg of nuclear extracts from chondrocytes treated with hyaluronan hexasaccharides (lanes 2 and 4) or untreated chondrocytes (lanes 1 and 3). Arrows denote the band location of the relevant transcription factor. For competition analysis, an EMSA reaction was performed in the presence of excess unlabeled probe (lanes 3 and 4). Lane 5 shows the probes without addition of nuclear extracts. The reactions were analyzed in duplicate and performed twice using 2 separate nuclear extracts. Band intensities were quantified using Quantity One software.