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. Author manuscript; available in PMC: 2010 Jun 28.
Published in final edited form as: Arthritis Rheum. 2005 Mar;52(3):800–809. doi: 10.1002/art.20937

Figure 4.

Figure 4

Effects of hyaluronan hexasaccharides (HA6) on the expression of matrix metalloproteinase 3 (MMP-3), type II collagen (collagen II), and cartilage oligomeric matrix protein (COMP) mRNA in bovine articular chondrocytes. Reverse transcription products of total RNA from chondrocytes with or without HA6 treatment were amplified by using a specific primer set for each target gene. The ratio of increase in the sample relative to the untreated control was calculated from the crossing point using the modification of Equation 1 as described by Rasmussen et al (17). A, MMP-3 induction by HA6 at the various doses (0–500 μg/ml) for 24 hours of treatment. B, C, and D, Time-course response (from 6 to 48 hours) to 250 μg/ml HA6 by MMP-3, type II collagen, and COMP mRNA, respectively. Open bars = no HA6; solid bars = addition of HA6. Bars show the mean and SD.