Figure 3.

Transient replication of HPV16 mutants with LCR and E1-E2 deletions. (A) Schematic representation of PUCGFP comprising an EGFP in a pUC19 backbone. An entire sequence and partial fragments of HPV16 DNA shown in solid lines were inserted into PUCGFP at BamHI to generate WT and mutants designated ΔLCR, ΔE1-E2, ΔLCR-E2, and L1 as described in Materials and Methods. Replication efficiencies of the HPV16 WT and mutants are evaluated by a transient transfection assay. Autoradiograms of representative Southern blots show replicated DNAs from transient transfection assays in C33A (B) and 293 (B). The assay was performed as described in Figure 2 legend. Ten pg to 1 ng of linear plasmid containing the entire HPV16 DNA, was loaded on the left-most lanes, as copy number controls.