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. 2010 Jun 28;5(6):e11247. doi: 10.1371/journal.pone.0011247

Figure 3. Depletion of MK2 by morpholino injection induced homologous chromosome misalignment and bipolar spindle assembly defects.

Figure 3

(A) Samples from control and MK2 MO injection groups were collected to test the efficiency of MK2 depletion. A total of 200 oocytes were injected with control or MK2 morpholino and cultured for 6 h after arrest at the GV stage for 24 h in M2-containing 2.5 µM milrinone. (B) Percentage of polar body extrusion in the MK2 morpholino injected group (n = 196) and control morpholino injected group (n = 137). Data are presented as mean ± SE. Different superscripts indicate statistical difference (p<0.05). (C) Immunostaining of oocytes injected with control and MK2 morpholino. After injection, oocytes were incubated for 12 h after arrest at the GV stage for 24 h, followed by immunostaining with α-tubulin antibody (green), p-MK2 (purple) and PI labeling for DNA (red). Bar 20 µm. (D) The above oocytes were immunostained with γ- tubulin (green), p-MK2 (red) and labeled for DNA (blue). Bar 20 µm. (E) The above oocytes were immunostained with Plk1 (green) and labeled with PI (red). Bar 20 µm.