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. 2010 Jun 28;5(6):e11247. doi: 10.1371/journal.pone.0011247

Figure 4. MK2 inhibitor CMPD1 treatment impairs spindle organization and chromosome alignment in mouse oocytes.

Figure 4

(A) Samples from control and CMPD1 groups were collected to test the efficiency of the MK2 inhibitor. CMPD1, 200 oocytes were cultured in M16 medium with 30 µM CMPD1 for 10 h; Control, 200 oocytes were cultured in M16 medium with DMSO for 10h. (B) The rate of oocytes with first polar body in the control group (n = 267) and CMPD1-treated group (n = 318). Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. Different superscripts indicate statistical difference (p<0.05). (C) Spindle morphologies and chromosome alignment in oocytes cultured with DMSO or MK2 inhibitor CMPD1. GV oocytes were cultured in M16 medium with DMSO or with 30 µM CMPD1 for 12 h and then stained for p-MK2 (purple), α-tubulin (green) and DNA (red). Bar 20 µm. (D) GV oocytes were cultured in M16 medium with DMSO or with 30 µM CMPD1 for 8.5 h and then stained for p-MK2 (red), Plk1 (green) and DNA (blue). Bar 20 µm. (E) Chromosome spreading was performed in oocytes that had been cultured for 8.5 h or 12 h of DMSO treatment (MI) (MII) or for 12 h of CMPD1 treatment. Representative images of each sample are shown. Bar 10 µm.