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. 2010 Jun 28;5(6):e11336. doi: 10.1371/journal.pone.0011336

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Van Buul et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Analysis of Src, Yes and Fyn-kinase (SYF)-deficient (SYF^-/-) or SYF^-/- with reconstituted Src (SYF^-/-+Src) or control (CTRL) mouse embryonic fibroblast (MEF) cells by western blotting using antibodies against Src, Yes or Fyn. Tubulin was used for protein loading control. (B) Immunofluorescent images show localization of ICAM-1-GFP in filopodia in SYF^-/- or SYF^-/-+Src MEF cells. (C) Graph shows that the expression of Src had only minor effect on the ICAM-1 FRAP in the SYF^-/- cells. (D) αICAM-1 antibody coated beads (size 3 µm) were allowed to adhere for 30 minutes on ICAM-1-GFP transfected SYF^-/- or SYF^-/-+Src cells. Number of beads per ICAM-1-GFP-positive cell was counted. No difference in adhesion of these beads the MEF cells is measured. Experiment was carried out three times.