Figure 1. Immunoprecipitation of Stau2 isoforms.

(A) Schematic representation of Stau2 isoforms. The Stau2 gene generates four different isoforms of 62, 59, 56 and 52 kDa through differential splicing. Black, grey and white boxes represent double-stranded RNA-binding (dsRBD) consensus sequence having full, partial or no RNA-binding activity, respectively. Hatched boxes represent the tubulin-binding domain (TBD). (B) Immunoprecipitation of Stau2 isoforms from embryonic E17-18 rat brain extracts using two different polyclonal anti-Stau2 antibodies, L1 (St2-L1) and L2 (St2-L2). The specificity of these antibodies was previously reported [7]. Pre-immune (PI) sera were used as controls. The Stau2⁵⁶ isoform is not visible in these cell extracts. * represents a non-specific IgG band.