Figure 2. RNA-binding proteins are associated with Stau2 isoforms in mRNPs.

N2A cells were mock transfected (−) or co-transfected with plasmids coding for either Stau2⁵⁹-HA₃ (59) or Stau2^62- HA₃ (62) and plasmids coding for PABPC1-myc (A), YB1-CFP (B), hsc70-CFP (C) or hnRNP H1-myc (D) as indicated. Immunoprecipitation of Stau2-containing RNPs was performed with anti-HA antibody and the proteins detected on western blots using anti-HA, anti-myc or anti-GFP antibodies as needed. The experiments were done in the absence (-RNase) or presence (+RNase) of Microccocal nuclease to determine if the Stau2-protein association requires an RNA bridge. These results were representative of at least three experiments. Input (INPUT) of transfected proteins before immunoprecipitation is also shown to indicate that the tagged-proteins were well expressed in these cells.