Figure 4. The interaction between Stau2⁶² and hsc70 is abolished in the presence of ATP.

(A) N2A cells were co-transfected with plasmids coding for Stau2^62- HA₃ and hsc70-CFP as done for figure 2. Immunoprecipitation of Stau2-containing RNPs was performed with anti-HA antibody and the proteins detected on western blots using anti-HA or anti-GFP antibodies as needed. The experiment was done in the absence (−) or presence of either Microccocal nuclease (+RNase) or ATP (+ATP). These results are representative of at least three experiments. (B) As done for figure 3C, MBP and MBP-Stau2⁶² were immobilized on different lanes of a SPR sensor chip. GST-hsc70 or GST was injected for 3 minutes over the surfaces in the presence or absence of ATP. The baseline obtained with the MBP-coupled reference surface was subtracted from the sensorgram obtained from the MBP-Stau2⁶²-coupled surface and a typical result is shown.