Figure 5. Co-localization of endogenous Stau2 with PABPC1-myc, YB1-CFP, hsc70-CFP and hnRNP H1-myc in hippocampal neurons.

Neurons were transfected with plasmids coding for either PABPC1-myc (A), YB1-CFP (B), hsc70-CFP (C) or hnRNP H1-myc (D). Twenty four hours post-transfection, neurons were fixed and labeled with anti-myc or anti-GFP (green) and anti-Stau2 (red) antibodies. Left: Fluorescence microscopy of hippocampal neurons in culture. Scale bars: 5 µm. Right: Higher magnification of images showing protein localization in dendrites. The lower panels represent the superposition of both green and red signals. Scale bars: 2 µm.