Table 3.
Scaled up ZHX homeodomain purification yields and crystallization trials
| Protein | Construct OPPF_ | Yield/(mg/l) | Number of 96 well plates | Structure |
|---|---|---|---|---|
| ZHX1 | 1612 | 4.5 | 16 | |
| 1613 | 2.1 | 10 | ||
| 1615 | 2.3 | 14 | ||
| 1632 | 1.8 | 12 | ||
| 1633 | 2.3 | 6 | ||
| 1635 | 3.5 | 17 | ||
| 1636 | 5.9 | 11 | ||
| 2264 | 4.1 | 12 | ||
| 2265 | 3.9 | 32 | Yes | |
| ZHX2 | 2268 | 11.5 | 25 | |
| 2273 | 5.0 | 14 | Yes | |
| ZHX3 | 2274 | 2.5 | 8 | |
| 2279 | 6.3 | 8 |
An additional construct containing the double ZHX1 zinc-finger domain was expressed & protein purified leading to a three-dimensional structure determined using NMR [19].