Table 1.
Locus name | BAC consensus sequence ID | GenBank Accession no. | Repeat Array | Primer Sequence (5' - 3') | Obtained allele size range (bp) | Tm (oC) used | Na | HE | HO | HWE P value | PIC |
---|---|---|---|---|---|---|---|---|---|---|---|
MABN01 | MA4_008L021 | AC186748 | (AG)12 | F: CCACTGAAGCTGAAAGGAGG | 500-540 | 56 | 3 | 0.667828 | 0.875000 | 0.021000* | 0.577 |
R: GGATTGTAGGTGACGGGAGA | 56 | ||||||||||
MABN03 | MA4_008L021 | AC186748 | (TG)10 | F: TGGTTGTATGTTTGCTGGGA | 500-545 | 60 | 3 | 0.593590 | 0.850000 | 0.013500* | 0.504 |
R: CAAAGTGCTGGCATGAGAAA | 60 | ||||||||||
MABN06 | MA4_008L021 | AC186748 | (ATAC)3 | F: GCAACCATCAACCAAAAACC | 344-360 | 58 | 3 | 0.444872 | 0.200000 | 0.013500* | 0.365 |
R: TTTGCAAGAAAATCGTGCTG | 58 | ||||||||||
MABN07 | MA4_008L021 | AC186748 | (ATA)6 | F: TTTTGATCATCATATGGGTCG | 500-540 | 60 | 2 | 0.344948 | 0.428571 | 0.512000 | 0.258 |
R: AGAGGGAGAGCCAAAGTGGT | 60 | ||||||||||
MABN08 | MA4_008L021 | AC186748 | (GA)13 | F: TTACCGTAAACGGAGCCAAC | 260-290 | 58 | 3 | 0.637631 | 1.000000 | 0.000000* | 0.544 |
R: GAAATCGAGGAAAACCGACA | 58 | ||||||||||
MABN13 | MA4_111B014 | AC186954 | (CA)6 | F: CCTCAACGAAGCATACAGCA | 210-240 | 58 | 2 | 0.450980 | 0.647059 | 0.106500 | 0.351 |
R: CAGTCTGGGCTGACACAGAA | 58 | ||||||||||
MABN15 | MA4_111B014 | AC186954 | (ATTTT)3 | F: CCAACTTCCATTTGGCTTTT | 490-520 | 58 | 2 | 0.315912 | 0.380952 | 1.000000 | 0.258 |
R: CGCAGGCGACTTCTTACAGT | 58 | ||||||||||
MABN17 | MA4_111B014 | AC186954 | (TCT)14 | F: CCCATGCAACTACAACAACG | 200-245 | 60 | 4 | 0.732804 | 1.000000 | 0.125000 | 0.659 |
R: GGAACCACGTGTCCTGATCT | 60 | ||||||||||
MABN19 | MA4_106O017 | AC186747 | (TTTAT)3 | F: CTCCACCGCTGCAAATTAT | 330-380 | 60 | 4 | 0.750794 | 0.944444 | 0.003000* | 0.681 |
R: TTCATTTGATTGGAAAGTGGAA | 60 | ||||||||||
MABN20 | MA4_106O017 | AC186747 | (AC)7 | F: AAGAAGTGCAACAGATGGGC | 344-380 | 56 | 3 | 0.537179 | 0.550000 | 0.727500 | 0.454 |
R: GCCAAAGGAATCATGCTGTT | 56 | ||||||||||
MABN22 | MA4_106O017 | AC186747 | (AG)6 | F: GTCGCAGAGATCAAGGAACC | 490-510 | 58 | 2 | 0.507549 | 0.619048 | 0.392000 | 0.373 |
R: GGACCTCCTATGTTTGCTGC | 58 | ||||||||||
MABN23 | MA4_106O017 | AC186747 | (TTA)4 | F: TCGATCATTTGGCATCACAT | 350-500 | 60 | 4 | 0.723577 | 0.952381 | 0.015500* | 0.641 |
R: CCAGGTAGCGAAGACGAGAC | 60 | ||||||||||
MABN25 | MA4_106O017 | AC186747 | (TAT)9 | F: TTTCATGATTTGAGGAGCCC | 380-410 | 58 | 2 | 0.462304 | 0.684211 | 0.049500* | 0.348 |
R: CCCCACAAGTATGTTCCCAC | 58 | ||||||||||
MABN26 | MA4_106O017 | AC186747 | (CT)24 | F: GTGGGAACATACTTGTGGGG | 375-395 | 58 | 2 | 0.493612 | 0.047619 | 0.000000* | 0.359 |
R: ACGGAAAACCACAAGCAATC | 58 | ||||||||||
MABN27 | MA4_106O017 | AC186747 | (GAA)4 | F: GGATGCAAAGACGGACAAAT | 470-520 | 58 | 3 | 0.667828 | 0.714286 | 0.000000* | 0.575 |
R: TAATGGCTTTGCAACTGCTG | 58 | ||||||||||
MABN28 | MA4_106O017 | AC186747 | (GA)23 | F: TGGAGGTCTCAACCAAAACC | 390-410 | 60 | 2 | 0.480769 | 0.550000 | 0.639500 | 0.367 |
R: AGATTGGCTACTGTGGGTGG | 60 | ||||||||||
MABN29 | MA4_106O017 | AC186747 | (GAT)5 | F: ACCAGCCACTGGAATCAAAC | 350-385 | 60 | 3 | 0.600000 | 0.866667 | 0.069000 | 0.506 |
R: GTCTGCTGAAGAGCCAAACC | 60 | ||||||||||
MABN30 | MA4_106O017 | AC186747 | (ATTTT)3 | F: CAGCCGTTGATGTTCAAATG | 360-380 | 60 | 2 | 0.387097 | 1.000000 | 0.000500* | 0.321 |
R: CGTTACGGTGGATCGTCTTT | 60 | ||||||||||
MABN34 | MA4_106O017 | AC186747 | (CT)18 | F: TAGGTGAGAATGGGACGGAG | 330-355 | 58 | 3 | 0.661451 | 0.368421 | 0.000000* | 0.571 |
R: CAGTAGCAGCAACCTGGTGA | 58 | ||||||||||
MABN35 | MA4_106O017 | AC186747 | (CT)14 | F: CTGTCACCAGGTTGCTGCTA | 270-320 | 56 | 4 | 0.664103 | 0.450000 | 0.005500* | 0.569 |
R: CTTCCTTGGACCTTTCATCG | 56 |
Tm, annealing temperature used; Na, number of alleles per locus observed; HE, expected heterozygosity under Hardy-Weinberg expectations; HO, observed heterozygosity; H-W, P value for deviation from Hardy-Weinberg equilibrium, with *significant departure (P < 0.05) from HW equilibrium; PIC, Polymorphism Information Content