. 2010 May 27;3:148. doi: 10.1186/1756-0500-3-148

Table 1.

Characteristics of microsatellite loci isolated from M. acuminata Calcutta 4 and polymorphic across 21 M. acuminata accessions.

Locus name	BAC consensus sequence ID	GenBank Accession no.	Repeat Array	Primer Sequence (5' - 3')	Obtained allele size range (bp)	T_m(^oC) used	N_a	H_E	H_O	HWE P value	PIC
MABN01	MA4_008L021	AC186748	(AG)12	F: CCACTGAAGCTGAAAGGAGG	500-540	56	3	0.667828	0.875000	0.021000*	0.577
				R: GGATTGTAGGTGACGGGAGA		56
MABN03	MA4_008L021	AC186748	(TG)10	F: TGGTTGTATGTTTGCTGGGA	500-545	60	3	0.593590	0.850000	0.013500*	0.504
				R: CAAAGTGCTGGCATGAGAAA		60
MABN06	MA4_008L021	AC186748	(ATAC)3	F: GCAACCATCAACCAAAAACC	344-360	58	3	0.444872	0.200000	0.013500*	0.365
				R: TTTGCAAGAAAATCGTGCTG		58
MABN07	MA4_008L021	AC186748	(ATA)6	F: TTTTGATCATCATATGGGTCG	500-540	60	2	0.344948	0.428571	0.512000	0.258
				R: AGAGGGAGAGCCAAAGTGGT		60
MABN08	MA4_008L021	AC186748	(GA)13	F: TTACCGTAAACGGAGCCAAC	260-290	58	3	0.637631	1.000000	0.000000*	0.544
				R: GAAATCGAGGAAAACCGACA		58
MABN13	MA4_111B014	AC186954	(CA)6	F: CCTCAACGAAGCATACAGCA	210-240	58	2	0.450980	0.647059	0.106500	0.351
				R: CAGTCTGGGCTGACACAGAA		58
MABN15	MA4_111B014	AC186954	(ATTTT)3	F: CCAACTTCCATTTGGCTTTT	490-520	58	2	0.315912	0.380952	1.000000	0.258
				R: CGCAGGCGACTTCTTACAGT		58
MABN17	MA4_111B014	AC186954	(TCT)14	F: CCCATGCAACTACAACAACG	200-245	60	4	0.732804	1.000000	0.125000	0.659
				R: GGAACCACGTGTCCTGATCT		60
MABN19	MA4_106O017	AC186747	(TTTAT)3	F: CTCCACCGCTGCAAATTAT	330-380	60	4	0.750794	0.944444	0.003000*	0.681
				R: TTCATTTGATTGGAAAGTGGAA		60
MABN20	MA4_106O017	AC186747	(AC)7	F: AAGAAGTGCAACAGATGGGC	344-380	56	3	0.537179	0.550000	0.727500	0.454
				R: GCCAAAGGAATCATGCTGTT		56
MABN22	MA4_106O017	AC186747	(AG)6	F: GTCGCAGAGATCAAGGAACC	490-510	58	2	0.507549	0.619048	0.392000	0.373
				R: GGACCTCCTATGTTTGCTGC		58
MABN23	MA4_106O017	AC186747	(TTA)4	F: TCGATCATTTGGCATCACAT	350-500	60	4	0.723577	0.952381	0.015500*	0.641
				R: CCAGGTAGCGAAGACGAGAC		60
MABN25	MA4_106O017	AC186747	(TAT)9	F: TTTCATGATTTGAGGAGCCC	380-410	58	2	0.462304	0.684211	0.049500*	0.348
				R: CCCCACAAGTATGTTCCCAC		58
MABN26	MA4_106O017	AC186747	(CT)24	F: GTGGGAACATACTTGTGGGG	375-395	58	2	0.493612	0.047619	0.000000*	0.359
				R: ACGGAAAACCACAAGCAATC		58
MABN27	MA4_106O017	AC186747	(GAA)4	F: GGATGCAAAGACGGACAAAT	470-520	58	3	0.667828	0.714286	0.000000*	0.575
				R: TAATGGCTTTGCAACTGCTG		58
MABN28	MA4_106O017	AC186747	(GA)23	F: TGGAGGTCTCAACCAAAACC	390-410	60	2	0.480769	0.550000	0.639500	0.367
				R: AGATTGGCTACTGTGGGTGG		60
MABN29	MA4_106O017	AC186747	(GAT)5	F: ACCAGCCACTGGAATCAAAC	350-385	60	3	0.600000	0.866667	0.069000	0.506
				R: GTCTGCTGAAGAGCCAAACC		60
MABN30	MA4_106O017	AC186747	(ATTTT)3	F: CAGCCGTTGATGTTCAAATG	360-380	60	2	0.387097	1.000000	0.000500*	0.321
				R: CGTTACGGTGGATCGTCTTT		60
MABN34	MA4_106O017	AC186747	(CT)18	F: TAGGTGAGAATGGGACGGAG	330-355	58	3	0.661451	0.368421	0.000000*	0.571
				R: CAGTAGCAGCAACCTGGTGA		58
MABN35	MA4_106O017	AC186747	(CT)14	F: CTGTCACCAGGTTGCTGCTA	270-320	56	4	0.664103	0.450000	0.005500*	0.569
				R: CTTCCTTGGACCTTTCATCG		56

Tm, annealing temperature used; Na, number of alleles per locus observed; H_E, expected heterozygosity under Hardy-Weinberg expectations; H_O, observed heterozygosity; H-W, P value for deviation from Hardy-Weinberg equilibrium, with *significant departure (P < 0.05) from HW equilibrium; PIC, Polymorphism Information Content