Figure 4. Subcellular localization of AtCRT1a, CRT1b, and CRT3.
Root sections of Arabidopsis incubated with AtCRT1b (A and B), and CRT1a (C) peptide antibodies. A. AtCRT1b antibody labeling is indicated by white arrowheads. Scale bar = 0.2 µm. Insert show enlarged area of immunogold labeled area. Scale bar = 50 nm. B. The AtCRT1b and callose antibody labeling are indicated by white, and black arrowheads, respectively. Scale bar = 50 nm. C. AtCRT1a and callose antibody labeling is indicated by white, and black arrowheads, respectively. Scale bar = 100 nm. PD; Plasmodesmata, CW; Cell wall. Insert show enlarged area of immunogold labeled area; white arrow heads CRT1a, and black arrow heads Callose. Scale bar = 20 nm D. Transient expression of AtCRT3 tagged with a CFP in tobacco leaves. Fluorescence is indicated in light-blue, and chloroplasts in red. Scale bar = 500 nm. Arrow indicates nuclear envelope.
