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. 2010 Jun 28;5(6):e11347. doi: 10.1371/journal.pone.0011347

Figure 1. N- and C- terminus contact via aromatic interactions.

Figure 1

The presence of conserved F-W-Y aromatic clusters in thermostable BSX (A), BHX (B), and BFX (C) are shown. Lys36 of BSX was replaced with Gln46 in BHX and Gln36 in BFX. The SWISS-pdb generated models of F4A (D), W6A (E), Y343A (F), ΔY343 (G), ΔW6 (H), ΔF4 (I) and F4W (J) are also shown. The introduction of alanine clearly disrupts the F-W-Y aromatic cluster (D, E & F) whereas replacement of Phe4 with Trp keeps the aromatic cluster intact (J). The predicted aromatic interactions, cation-pi interactions, and optimal activity temperatures for BSX, BHX and BFX are presented in tabular form (K).