Figure 4. Effect of Proteinase K.

The SDS-PAGE profiles of all proteins in the absence (A) and presence (B) of proteinase K. Samples were incubated with proteinase K for 24 hours at 301K. Note that all samples except W6A, ΔF4 and Y343A showed proteinase K resistance. The SDS-stability profiles of protein samples subjected to SDS-PAGE with (C) or without (D) prior boiling for 15 min are shown. All samples were incubated for 15 min in the presence of loading buffer containing SDS before they were subjected to SDS-PAGE.