Figure 5. Murine cytomegalovirus (MCMV) immediate early 1 (IE-1) gene sequence comparison.
PCR amplicons for IE-1 (384 bp) were sequenced by PCR gel isolation. DNA sequences from chow MCMV, wild MCMV, and vivarium MCMV were aligned and compared to lab MCMV (Smith strain). Shown is a 40bp sequence that highlights similarities and differences between the MCMV isolates. Dark gray indicates differences unique to Wild MCMV, and light gray differences seen in vivarium-acquired MCMV. Sequencing results were confirmed in triplicate (not shown).
