TABLE 2.
RAD9-interacting genes
| Gene | Classe | Viability for single (%) (xxxΔ)a | Viability for double (%) (xxxΔ rad9Δ)a | Ratiob | p valuec |
|---|---|---|---|---|---|
| RAD5 | Error-free bypass | 0.016±0.01 | 0d | ∞d | NDd |
| UBC13 | Error-free bypass | 12±2 | 0.023±0.02 | 357.7 | 0.0015 |
| REV7 | Error-prone bypass | 31±3 | 0.037±0.04 | 223.1 | 0.0015 |
| MMS2 | Error-free bypass | 15±1 | 0.078±0.012 | 172.8 | 0.0011 |
| REV1 | Error-prone bypass | 60±21 | 0.12±0.10 | 64.7 | 0.0016 |
| RAD54 | HR | 12±6 | 0.17±0.11 | 47.4 | 0.0016 |
| REV3 | Error-prone bypass | 76±15 | 0.20±0.12 | 40.8 | 0.0016 |
| MMS1 | HR intermediate resolution | 54±8 | 0.25±0.05 | 33.0 | 0.0016 |
| ELG1 | Clamp loader | 28±1 | 0.37±0.22 | 22.4 | 0.0018 |
| DDC1 | 9-1-1 complex | 12±2 | 0.41±0.10 | 20.4 | 0.0017 |
| MGT1 | Methyltransferase | 39±6 | 0.42±0.16 | 19.9 | 0.0018 |
| MUS81 | HR intermediate resolution | 2.7±0.4 | 0.14±0.05 | 18.9 | 0.0040 |
| MMS4 | HR intermediate resolution | 3.6±0.2 | 0.20±0.09 | 17.8 | 0.0011 |
| ISW1 | Chromatin | 41±12 | 0.52±0.17 | 16.1 | 0.0018 |
| POT1 | Fatty acid oxidation | 34±14 | 0.54±0.41 | 15.4 | 0.0022 |
| RAD17 | 9-1-1 complex | 6±0.7 | 0.43±0.09 | 14.2 | 0.0028 |
| SGS1 | HR intermediate resolution | 28±5 | 0.67±0.23 | 12.5 | 0.0020 |
| RAD55 | HR | 18±8 | 0.70±0.33 | 12.0 | 0.0021 |
| BBC1 | Actin patch | 84±24 | 0.73±0.65 | 11.4 | 0.0030 |
| IXR1 | Chromatin | 29±10 | 0.99±0.29 | 8.4 | 0.0022 |
| YIL158W | unknown | 60±11 | 1.3±0.8 | 6.3 | 0.0047 |
| ESC2 | HR intermediate resolution | 18±5 | 1.3±0.9 | 6.1 | 0.0050 |
| UIP5 | unkown | 29±4 | 1.4±0.3 | 5.8 | 0.0027 |
| PSY3 | Error-free bypass | 30±11 | 1.5±0.5 | 5.5 | 0.0035 |
| MSN1 | Transcription | 24±6 | 1.6±0.8 | 5.0 | 0.0048 |
Cells were exposed to 0.01% MMS in liquid rich medium for 5 hours. Wild type is BY4741 (S288C). Error represents the standard deviation calculated for three independent biological experiments performed on three subsequent days on three independent segregants for each double mutant. After MMS treatment, the survival rate for wild type and rad9Δ strains are 76±13% and 8.3±0.8%.
Ratio is calculated as the viability of the most sensitive single mutant (i.e. either rad9Δ or xxxΔ) versus the viability of the rad9Δ xxxΔ double mutant.
The p-values for the reliability of the difference of survival rates between the single and double mutants were calculated using t-tests.
There were no viable colonies for the rad5Δ rad9Δ double mutant after 5 hour exposure to 0.01% MMS; 2 × 104 cells were plated; hence, viability was ≤ 0.005%. (p-value can not be accurately calculated.)
Gene functions are classified according to annotations in Saccharomyces Genome Database (www.yeastgenome.org) and the literature referenced in the database (see Supplemental table S1). PSY3 was very recently assigned to the error-free bypass branch of PRR pathway by Ball et al[56].